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Comment
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Following the incubation, Fluorescent Substrate (100 nM) was added to each well and the fluorescent intensity was measured (ex 485 nm, em 535 nm) every two minutes. The data was plotted as a kinetic graph (Figure 1). Fluorescent intensity for the 1.25 μg reaction at a 60 minute kinetic reading should have 10-fold higher signal than the initial 0 minute kinetic reading. Lot 10510001 had a 22-fold increase in fluorescent intensity. There should be no significant increase in fluorescence for the wells treated with inhibitor.
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