產品詳情
簡單介紹:
anti-CD163antibody(CD163Molecule)(PE)
詳情介紹:
Immunogen | Hairy cell leukemia cells |
Clone | GHI-61 |
Isotype | IgG1 |
Specificity | This antibody recognizes CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex. |
Cross-Reactivity (Details) | Species reactivity (tested):Human |
Purification | Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. |
Alternative Name | CD163 (CD163 Antibody Abstract) |
Background | CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130 |
Gene ID | 9332 |
NCBI Accession | NP_004235 |
UniProt | Q86VB7 |
Research Area | Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells |
Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
Buffer | Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2?% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | DO NOT FREEZE! This product is photosensitive and should be protected from light. |
Storage | 4 °C |
Background publications |
Kusi, Gyan, Goka, Dodoo, Obeng-Adjei, Troye-Blomberg, Akanmori, Adjimani: "Levels of soluble CD163 and severity of malaria in children in Ghana." in: Clinical and vaccine immunology : CVI, Vol. 15, Issue 9, pp. 1456-60, 2008 (PubMed).
Bover, Cardó-Vila, Kuniyasu, Sun, Rangel, Takeya, Aggarwal, Arap, Pasqualini: "A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 12, pp. 8183-94, 2007 (PubMed). Kim, Alvarez, Fisher, Bronfin, Westmoreland, McLaurin, Williams: "CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood." in: The American journal of pathology, Vol. 168, Issue 3, pp. 822-34, 2006 (PubMed). Moniuszko, Kowal, Rusak, Pietruczuk, Dabrowska, Bodzenta-Lukaszyk: "Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants." in: Clinical and vaccine immunology : CVI, Vol. 13, Issue 6, pp. 704-7, 2006 (PubMed). Madsen, M?ller, Nielsen, Jacobsen, Graversen, van den Berg, Moestrup: "Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 51561-7, 2004 (PubMed). Law, Micklem, Shaw, Zhang, Dong, Willis, Mason: "A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily." in: European journal of immunology, Vol. 23, Issue 9, pp. 2320-5, 1993 (PubMed). Pulford, Micklem, McCarthy, Cordell, Jones, Mason: "A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4." in: Immunology, Vol. 75, Issue 4, pp. 588-95, 1992 (PubMed). |