Purpose | L-Series Mouse Antibody Array 308 Glass Slide Kit. Detects 308 Mouse Proteins. Suitable for serum, plasma and cell culture supernatants. |
Brand | RayBio? |
Sample Type | Plasma, Cell Culture Supernatant, Serum |
Analytical Method | Semi-Quantitative |
Detection Method | Fluorometric |
Specificity | 6Ckine, Activin A, Activin C, Activin RIB / ALK-4, Adiponectin / Acrp30, AgRP, ALCAM, Angiopoietin-like 2, Angiopoietin-like 3, AREG (Amphiregulin), Artemin, Axl, bFGF, B7-1/CD80, BAFF R / TNFRSF13C, BCMA / TNFRSF17, beta-Catenin, BLC, BTC (Betacellulin), Cardiotrophin-1, CCL1 / I-309 / TCA-3, CCL28, CCL4 / MIP-1 beta, CCL7 / MCP-3 / MARC, CCL8 / MCP-2, CCR10, CCR3, CCR4, CCR6, CCR7, CCR9, CD11b, CD14, CRP, CD27 / TNFRSF7, CD27 Ligand / TNFSF7, CD30, CD30 L, CD40, CD40 Ligand / TNFSF5, Cerberus 1, Chordin-Like 2, Coagulation Factor III / Tissue Factor, Common gamma Chain / IL-2 R gamma, CRG-2, Cripto, Crossveinless-2, Cryptic, Csk, CTACK , CTLA-4 / CD152, CXCL14 / BRAK, CXCL16, CXCR2 / IL-8 RB, CXCR3, CXCR4, CXCR6, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, DPPIV / CD26, DR3 / TNFRSF25, Dtk, EDAR, EGF R, EG-VEGF / PK1, Endocan, Endoglin / CD105, Endostatin, Eotaxin, Eotaxin-2, Epigen, Epiregulin, Erythropoietin (EPO), E-Selectin, FADD, FAM3B, Fas / TNFRSF6, Fas Ligand, FCrRIIB / CD32b, FGF R3, FGF R4, FGF R5 beta, FGF-21, Fit-3 Ligand, FLRG (Follistatin), Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-6, Frizzled-7, Galectin-3, G-CSF, GDF-1, GDF-3, GDF-5, GDF-8, GDF-9, GFR alpha-2 / GDNF R alpha-2, GFR alpha-3 / GDNF R alpha-3, GFR alpha-4 / GDNF R alpha-4, GITR, GITR Ligand / TNFSF18, Glut2, GM-CSF, Granzyme B, Granzyme D, Granzyme G, Gremlin, Growth Hormone R, HGF R, HGF, HVEM / TNFRSF14, ICAM-1, ICAM-2 / CD102, ICAM-5, ICK, IFN-alpha / beta R1, IFN-alpha / beta R2, IFN-beta, IFN-gamma , IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGFBP-rp1 / IGFBP-7, IGF-I, IGF-II, IL-1 alpha, IL-1 beta, IL-1 R4 / ST2, IL-1 R6 / IL-1 R rp2, IL-1 R9, IL-1 RI, IL-1 RII, IL-2, IL-2 R alpha, IL-2 R beta, IL-3, IL-3 R alpha, IL-3 R beta, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-9, IL-9 R, IL-10, IL-10 R alpha, IL-11, IL-12 p40/p70, IL-12 p70, IL-12 R beta 1, IL-13, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 R alpha/IL-1 R5, IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-28 / IFN-lambda, IL-31, IL-31 RA, Insulin, Integrin beta 2 / CD18, I-TAC, KC, Kremen-1, Kremen-2, Lefty-1, Leptin R, LEPTIN(OB), LIF, LIGHT / TNFSF14, LIX, LRP-6, L-Selectin, Lungkine, Lymphotactin, Lymphotoxin beta R / TNFRSF3, MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MFRP, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta , MMP-2, MMP-3, MMP-9, MMP-12, MMP-14 / LEM-2, MMP-24 / MT5-MMP, Neuregulin-3 / NRG3, Neurturin, NGF R / TNFRSF16, NOV / CCN3, Osteoactivin / GPNMB, Osteopontin, Osteoprotegerin, OX40 Ligand / TNFSF4, PDGF C, PDGF R alpha, PDGF R beta, Pentraxin3 / TSG-14, PF-4, PlGF-2, Progranulin, Prolactin, P-Selectin, RAGE, RANTES, RELM beta, Resistin, S100A10, SCF, SCF R / c-kit, SDF-1, Serum Amyloid A1, Shh-N, SIGIRR, SLPI, Soggy-1, SPARC, Spinesin Ectodomain, TACI / TNFRSF13B, TARC, TCA-3, TCCR / WSX-1, TECK, TFPI, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta RI / ALK-5, TGF-beta RII, Thrombospondin, Thymus Chemokine-1, Tie-2, TIMP-1 , TIMP-2, TIMP-4, TL1A / TNFSF15, TLR1, TLR2, TLR3, TLR4, TMEFF1 / Tomoregulin-1, TNF RI / TNFRSF1A, TNF RII, TNF-alpha, TNF-beta / TNFSF1B, TPO, TRAIL / TNFSF10, TRAIL R2 / TNFRSF10B, TRANCE / TNFSF11, TREM-1, TROY, TSLP, TSLP R, TWEAK / TNFSF12, TWEAK R / TNFRSF12, Ubiquitin, uPAR, Urokinase, VCAM-1, VE-Cadherin, VEGF, VEGF R1, VEGF R2, VEGF R3, VEGF-B, VEGF-C, VEGF-D, WIF-1, WISP-1 / CCN4 |
Characteristics |
|
Components |
L-Series Antibody Array Membranes or Glass Slides Spin Columns / Dialysis Tube Labeling Reagent Stop Solution Blocking Buffer Streptavidin-Conjugated HRP or Streptavidin-Conjugated HiLyte Fluor 532 Wash Buffer 1 Wash Buffer 2 Plastic Sheets Floating Dialysis Rack Plastic Incubation Tray Detection Buffer C Detection Buffer D *Accessories include: 2-well or 4-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film |
Material not included |
Distilled or de-ionized water KCl, NaCl, KH2PO4 and Na2HPO4 Small plastic or glass containers Orbital shaker or oscillating rocker Beaker, stir plate and stir bar 1?mL tube Pipettors, pipette tips and other common lab consumables Laser scanner for fluorescence detection (list of compatible scanners available at http://www.raybiotech.com/resources.asp) Aluminum foil |
Application Notes | Completely cover membranes with sample or buffer during incubation and cover Plastic Incubation Tray with lid to avoid drying. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rotation. Several incubation steps such as step 3 in page 10 (sample incubation) or step 7 in page 11 (HRP-Conjugated Streptavidin incubation) may be done at 4?°C for overnight. |
Comment |
The L-series arrays utilize direct labeling for signal detection, wherein the antigen is tagged with biotin prior to incubation with the capture antibody. The signal is then developed with a streptavidin-conjugated HRP or fluor. Since this array requires only a single antibody per target molecule (as opposed to an antibody pair), any possibility of interactions between antibodies within the same array panel is eliminated. Thus, an unlimited number of antibodies may theoretically be included in each panel, making this array platform ideal for high-content screening of protein expression. |
Sample Volume | 100 μL |
Plate | Membrane,Glass Slide |
Protocol |
|
Sample Preparation |
Preparation of Samples |
Assay Procedure |
Blocking and Incubation |
Calculation of Results |
To obtain optimal results, it is suggested to try several different exposure times until the best one is determined. Then, by comparing the signal intensities, relative expression levels of the target proteins can be made. The intensities of signals can be quantified by densitometry. Anti-HRP (P-1a, P-2a, P-3a) and antistreptavidin (P-1b, P-2b, P-3b) will produce positive control signals, which can be used to identify the orientation and help normalize the results from different arrays being compared. Antibody affinity to its target varies significantly between antibodies. The intensity detected on the array with each antibody depends on this affinity; therefore, signal intensity comparison can be performed only within the same antibody/antigen system and not between different antibodies. |
Restrictions | For Research Use only |
Handling Advice | Always use forceps to handle membranes and grip the membranes by the edges only. Never allow membranes to dry during the experiment. Avoid touching membranes with hands or any sharp tools. |
Storage | 4 °C/-20 °C |
Storage Comment | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Expiry Date | 6 months |
Product cited in: |
Guo, Miller, Pencina, Wong, Lee, Yee, Toraldo, Jasuja, Bhasin: "Joint dysfunction and functional decline in middle age myostatin null mice." in: Bone, Vol. 83, pp. 141-8, 2016 (PubMed).
Kano: "Tumor cell secretion of soluble factor(s) for specific immunosuppression." in: Scientific reports, Vol. 5, pp. 8913, 2015 (PubMed). Zhi-Feng, Le-Yuan, Xiao-Hui, Ya-Bo, Jian-Ying, Yong, Zhao-Chong: "TLR4-dependent immune response promotes radiation-induced liver disease by changing the liver tissue interstitial microenvironment during liver cancer radiotherapy." in: Radiation research, Vol. 182, Issue 6, pp. 674-82, 2014 (PubMed). Ding, Yuan, Liu, Mao, Zhao, Huang, Zhang, Fang, Song, Yuan, Xie, Liu, Liu: "Protective effects of astragaloside IV on db/db mice with diabetic retinopathy." in: PLoS ONE, Vol. 9, Issue 11, pp. e112207, 2014 (PubMed). Tang, Zhu, Cheng, Chen, Zhang, Sun, Kishore, Phillips, Losordo, Qin: "Hypoxic preconditioning enhances the benefit of cardiac progenitor cell therapy for treatment of myocardial infarction by inducing CXCR4 expression." in: Circulation research, Vol. 104, Issue 10, pp. 1209-16, 2009 (PubMed). Choi, Gergis, Sokoll: "The effects of d-tubocurarine, pancuronium and atracurium on the responses of gastrocnemius and soleus muscles in the cat." in: Acta anaesthesiologica Scandinavica, Vol. 28, Issue 6, pp. 608-11, 1985 (PubMed). |