產品詳情
簡單介紹:
HumanIgGisotypecontrol(Biotin)
詳情介紹:
Isotype | IgG |
Characteristics | Concentration Definition: by UV absorbance at 280 nm |
Sterility | Sterile filtered |
Background |
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin. Synonyms: Human immunoglobulin G, Vitamin H, coenzyme R |
Research Area | Immunology, Secondary Antibodies |
Application Notes | Human IgG whole molecule Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments. |
Restrictions | For Research Use only |
Format | Lyophilized |
Reconstitution | Restore with deionized water (or equivalent) |
Concentration | 1.0 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Aliquot to Avoid repeated freezing and thawing. |
Storage | 4 °C |