產品詳情
簡單介紹:
Z-VAD-FMK
詳情介紹:
Purpose | A pan-caspase inhibitor |
Sequence | Z-Val-Ala-Asp(OMe)-FMK |
Characteristics | A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups). |
Purity | Single spot by TLC |
Chemical Name | Z-VAD-FMK, Z-VAD-fluoromethylketone, Caspase-Family Inhibitor (fluoromethylketone) |
Formula | C??H??N?O?F |
Permeability | Cell-permeable |
Solubility | DMSO (20 mM) |
Molecular Weight | 467.5 g/mol |
CAS-No | 187389-52-2 |
Application Notes |
We recommend using 2-10 μM for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions. |
Restrictions | For Research Use only |
Format | Solid, Lyophilized |
Handling Advice | Protect from light and moisture |
Storage | -20 °C |
Expiry Date | 12 months |
Supplier Images |
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Product cited in: |
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Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed). Xie, Jiang, Wan, Du, Guo, Liu, Ye, Niu, Wu, Dong, Zhang: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011 (PubMed). Dolai, Pal, Yadav, Adak: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed). Ariyama, Kono, Matsuda, Inoue, Arai: "Decrease in membrane phospholipid unsaturation induces unfolded protein response." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22027-35, 2010 (PubMed). Fukazawa, Maeda, Matsuoka, Yamatsuji, Shigemitsu, Morita, Faiola, Durbin, Soucek, Naomoto: "Inhibition of Myc effectively targets KRAS mutation-positive lung cancer expressing high levels of Myc." in: Anticancer research, Vol. 30, Issue 10, pp. 4193-200, 2010 (PubMed). Gao, Bauvy, Souquère, Tonelli, Liu, Zhu, Qiao, Bakula, Proikas-Cezanne, Pierron, Codogno, Chen, Mehrpour: "The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25570-81, 2010 (PubMed). Saha, Jiang, Mukai, Chang: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010 (PubMed). Ito, Yip, Katz, Fonseca, Hedley, Chow, Xu, Wood, Bastianutto, Schimmer, Kelley, Liu: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed). Liu, Deng, Lancto, Abrahamsen, Rutherford, Enomoto: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed). Dai, Liu, Tang, DeSano, Burstein, Davis, Pienta, Lawrence, Xu: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed). 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