產品詳情
簡單介紹:
Z-YVAD-FMK
詳情介紹:
Purpose | A caspase-4 inhibitor |
Sequence | Z-Tyr-Val-Ala-Asp(OMe)-FMK |
Characteristics | A synthetic peptide that irreversibly inhibits activity of caspase-1. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups). |
Purity | Single spot by TLC |
Chemical Name | Z-YVAD-FMK, Z-YVAD-fluoromethylketone, Caspase-1 Inhibitor (fluoromethylketone) |
Formula | C??H??FN?O? |
Permeability | Cell-permeable |
Solubility | DMSO (20 mM) |
Molecular Weight | 630.7 g/mol |
Application Notes |
We recommend using 2-10 μM for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions. |
Restrictions | For Research Use only |
Format | Solid, Lyophilized |
Handling Advice | Protect from light and moisture |
Storage | -20 °C |
Expiry Date | 12 months |
Supplier Images |
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Product cited in: |
Zhou, Shah, Yang, Zhang, Zhou, Zhao: "Virulent Mycobacterium bovis Beijing Strain Activates the NLRP7 Inflammasome in THP-1 Macrophages." in: PLoS ONE, Vol. 11, Issue 4, pp. e0152853, 2016 (PubMed).
Burm, Zuiderwijk-Sick, t Jong, van der Putten, Veth, Kondova, Bajramovic: "Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 2, pp. 678-87, 2015 (PubMed). Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014 (PubMed). Tseng, Thein, Kinnunen, Lashkari, Gregory, DAmore, Ksander: "NLRP3 inflammasome activation in retinal pigment epithelial cells by lysosomal destabilization: implications for age-related macular degeneration." in: Investigative ophthalmology & visual science, Vol. 54, Issue 1, pp. 110-20, 2013 (PubMed). Lee, Hwang, Palaniyar, Grinstein, Philpott, Hu: "Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection." in: PLoS ONE, Vol. 7, Issue 4, pp. e35812, 2012 (PubMed). Chang, Lin, Wu, Kuo, Wu, Shieh, Gean, Chen, Chen, Wu, Tai, Tseng: "Mechanisms underlying benzyl alcohol cytotoxicity (triamcinolone acetonide preservative) in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 52, Issue 7, pp. 4214-22, 2011 (PubMed). Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed). Shao: "Understanding the mechanisms of human tubal ectopic pregnancies: new evidence from knockout mouse models." in: Human reproduction (Oxford, England), Vol. 25, Issue 3, pp. 584-7, 2010 (PubMed). Wei, Kadia, Tong, Zhang, Jia, Yang, Hu, Tambaro, Viallet, OBrien, Garcia-Manero: "The combination of a histone deacetylase inhibitor with the Bcl-2 homology domain-3 mimetic GX15-070 has synergistic antileukemia activity by activating both apoptosis and autophagy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 15, pp. 3923-32, 2010 (PubMed). |