產品詳情
簡單介紹:
Caspase-10InhibitorAEVD-FMK
詳情介紹:
Purpose | A caspase-10 inhibitor |
Sequence | Z-Ala-Glu(OMe)-Val-Asp(OMe)-FMK |
Characteristics |
PubChem CID: 44135225 A synthetic peptide inhibitor that inhibits caspase-10 and related caspase activity. FMK inhibitors are irreversible and non-toxic, and can be used in both in vivo and in vitro studies. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups). |
Purity | > 95?% by HPLC |
Chemical Name | Z-AEVD-FMK, Caspase-10 Inhibitor (fluoromethylketone) |
Formula | C??H??N?O??F |
Permeability | Cell-permeable |
Solubility | In DMSO (10 mM) |
Molecular Weight | 610.63 g/mol |
Application Notes | We recommend using 1000-5000X dilutions for inhibiting caspase-10 and related caspase activity (e.g., Add 1 μL to 1-5 mL of culture medium). The optimal doses may vary for different cells and culture conditions. The inhibitor is designed as a methyl ester to facilitate cell permeability. |
Restrictions | For Research Use only |
Format | Liquid |
Concentration | 10 mM |
Handling Advice | Protect from light and moisture |
Storage | -20 °C |
Expiry Date | 12 months |
Product cited in: |
Appelqvist, W?ster, Eriksson, Rosdahl, Ollinger: "Lysosomal exocytosis and caspase-8-mediated apoptosis in UVA-irradiated keratinocytes." in: Journal of cell science, Vol. 126, Issue Pt 24, pp. 5578-84, 2013 (PubMed).
Westphal, Sytnyk, Schachner, Leshchynska: "Clustering of the neural cell adhesion molecule (NCAM) at the neuronal cell surface induces caspase-8- and -3-dependent changes of the spectrin meshwork required for NCAM-mediated neurite outgrowth." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 42046-57, 2010 (PubMed). Klumpp, Rycyk, Chen, Thumbikat, Sengupta, Schaeffer: "Uropathogenic Escherichia coli induces extrinsic and intrinsic cascades to initiate urothelial apoptosis." in: Infection and immunity, Vol. 74, Issue 9, pp. 5106-13, 2006 (PubMed). Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed). Ruckdeschel, Mannel, Schr?ttner: "Divergence of apoptosis-inducing and preventing signals in bacteria-faced macrophages through myeloid differentiation factor 88 and IL-1 receptor-associated kinase members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 9, pp. 4601-11, 2002 (PubMed). Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed). |