|
Characteristics
|
Reaction of the IRDye 680RD Azide group with the alkyne group is typically accomplished by copper-catalyzed Click Chemistry, whereas the IRDye 680RD Azide group spontaneously reacts with the DBCO group over a wide range of conditions. Within physiologically-relevant temperature and pH ranges, IRDye 680RD Azide is inert toward naturally occurring nucleophiles such as amines and hydroxyls. However, the azide group is unstable toward sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).
|
