產(chǎn)品詳情
簡單介紹:
GSTSelector
詳情介紹:
Sample Type | Cell Extracts |
Specificity | The Antibody with Clone G19 recognizes Schistosoma japonicum Glutathione S-transferase (GST) |
Characteristics | GST Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4?% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GST Selector thus features high affinity and superior capacity for GST fusion proteins while showing negligible non-specific background. GST Selector is compatible not only with physiological buffers but also with high stringency buffers. GST Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs. |
Components | 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody |
Material not included | wash buffers, columns, tubes |
ProductDetails: Bead Ligand | Antibody |
ProductDetails: Bead Matrix | Agarose beads |
ProductDetails: Bead Size | 90 μm |
Alternative Name | Glutathione S-transferase (GST) |
Background | Glutathione S-transferase (GST) from Schistosoma japonicum is a 26 kDa enzyme, often found as a fusion partner in expression systems. GST folds rapidly and boosts the expression and the folding of the fusion partner. It is also used as purification tag wi |
Application Notes | 200?μL slurry for up to 10 reactions |
Comment |
Capacity: > 4 μg GST per μl of packed beads (i.e. 2 μl of slurry) |
Restrictions | For Research Use only |
Buffer | 50?% slurry in 20?% ethanol / PBS 0.09?% sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C |